WebThe user need to give them different names, for example, -–exptCond1 CTCF_cond1.bed -–exptCond2 CTCF_cond2.bed. For multiple replicates of same condition, you can specify multiple replicates as separate files, for example, -–exptCond1 CTCF_cond1_rep1.bed -–exptCond1 CTCF_cond1_rep2.bed (note that they need to have the same name). GEM ... WebJan 7, 2024 · The architectural protein CTCF is a mediator of chromatin conformation, but how CTCF binding to DNA is regulated remains poorly understood. Here the authors find that there is a shared subset of ...
Elevated enhancer-oncogene contacts and higher oncogene
WebIn the present study, the effect was investigated of CTCF activity on PAX6 transcription through interaction with five essential motifs located in an 80-bp region upstream from the PAX6 P0 promoter. Methods: An electrophoretic mobility shift assay (EMSA) was used to determine the interaction between CTCF and DNA binding motifs. WebSep 13, 2024 · CTCF absence leads to loss of chromatin structure and affects the expression of thousands of genes, including many developmental regulators. ... Conversion of BED to BigWig files was performed ... biodiversity of aquatic insects
TAD cliques predict key features of chromatin organization
WebAug 24, 2024 · Acute CTCF depletion alters chromatin accessibility. Although many crude loss-of-function models targeting CTCF have been extensively studied [17, 23,24,25,26,27,28], accumulated secondary effects were inevitably observed.The acute protein degradation system was recently developed as an essential tool to study direct … The primary role of CTCF is thought to be in regulating the 3D structure of chromatin. CTCF binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures like the nuclear lamina. It also defines the boundaries between active and heterochromatic DNA. Since the … See more Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, … See more CCCTC-Binding factor or CTCF was initially discovered as a negative regulator of the chicken c-myc gene. This protein was found to be binding to three regularly spaced repeats of … See more CTCF binds to the consensus sequence CCGCGNGGNGGCAG (in IUPAC notation). This sequence is defined by 11 zinc finger motifs in its structure. CTCF's binding is disrupted by See more • Ohlsson R, Renkawitz R, Lobanenkov V (2001). "CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease". Trends Genet. 17 (9): 520–7. doi:10.1016/S0168-9525(01)02366-6. PMID 11525835. • Klenova EM, Morse … See more The binding of CTCF has been shown to have many effects, which are enumerated below. In each case, it is unknown if CTCF directly evokes the outcome or if it does so indirectly (in particular through its looping role). Transcriptional … See more CTCF binds to itself to form homodimers. CTCF has also been shown to interact with Y box binding protein 1. CTCF also co-localizes with See more • CCCTC-binding+factor at the U.S. National Library of Medicine Medical Subject Headings (MeSH) • FactorBook CTCF See more WebApr 9, 2024 · NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data. biodiversity ngo