WebMar 10, 2010 · Recently, a novel method for detection of DNA synthesis has been developed based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, into cellular DNA and the subsequent reaction of EdU with a fluorescent azide in a copper-catalyzed [3+2] cycloaddition ("Click" reaction). … WebFeb 28, 2024 · The coverslips were incubated with a primary antibody against EdU (RiboBio, Guangzhou, China) at 4 ℃ overnight, followed by a secondary antibody against Apollo488 for 1 hour at room temperature. Images were captured under a fluorescence microscope. Cell cycle analysis. Cell cycle was analyzed by flow cytometry.

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WebApr 11, 2024 · Colony forming ability was assessed by a colony formation assay. Cell proliferation was evaluated and measured by using the 5-ethynyl-2′-deoxyuridine (EdU) assay kit (RiboBio, China). Each group has three biological replicates. Detailed vitro experimental protocol can be found in the supplemental material . 2.12 Statistical analysis WebEdU培养基 100 μL 200 μL 300 μL 500 μL 1 mL 2 mL 染色反应液 100 μL 200 μL 300 μL 500 μL 1 mL 2 mL 注：1）*表示贴壁细胞通常采用的培养容器，EdU 培养基与染色反应液用 … sandy haven pembrokeshire cottages

EdU（5-乙炔基-2’-脱氧尿苷） - Thermo Fisher

WebEdU (5-ethynyl-2’-deoxyuridine), a thymidine analog that is an alternative to BrdU, is also used in DNA proliferation assays that are simpler and faster than the BrdU assay. NB: EdU is also available as free molecule as ab146186 (EdU). In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample. WebMay 1, 2024 · The Cell counting kit-8 (CCK-8) (Biotechwel, #WH1199) and EdU (RiboBio, #C10310-1) assays were performed according to the manufacturers' instructions. Briefly, after transfected with ALKBH5 siRNA or infected with the ALKBH5 adenovirus, CMECs were reseeded into a 96-well plate (5000 cells/ well), followed by the normoxic or hypoxic … WebNov 6, 2024 · Cell proliferation in the root meristem was visualized by the incorporation of EdU into DNA at S-phase (Kotogany et al. 2010). EdU staining was performed using an EdU kit (C10310, Apollo 488, Ribobio) according to Li et al. . Briefly, roots of 5-day-old rice seedlings (10 seedlings for each genotype) were immersed in a 50 μM EdU solution for 2 h. sandy hawthorne aurora ohio