WebJan 31, 2024 · Protein expression was induced by the addition of IPTG (Biosynth, Sankt Gallen, Switzerland) at a final concentration of 0.5 mM. ... (Sigma) was added slowly to a total concentration of 0.5 g/100 mL lysate. DNase (Thermo Scientific, Waltham, MA, USA) and 10 mM MnCl2 were added to the supernatant and the reaction was incubated at 37 °C … WebThermo Scientific Chemicals Synonym (s): IPTG Formula: C9H18O5S Formula weight: 238.30 Purity: 99% CAS Number: 367-93-1 Harmonized Tariff Code: 2932.99 Iptg Compare this item Isopropyl-B-D-Thiogalactopyranoside INDOFINE Chemical Company MF: C9H18O5S MF: 238.3 CAS: 367-93-1 Purity: >99% by HPLC Compare this item
Protein Expression Protocol Popa Lab
WebBriefly, bacterial cells were lysed with B-Per extraction reagent (Thermo Scientific , #78248) supplemented with DNAse I (Thermo Scientific, #90083) and protease inhibitor cocktail (Thermo ... Web367-93-1 - Isopropyl-beta-D-thiogalactopyranoside (IPTG), dioxane free, 99+%, Ultrapure, Thermo Scientific - IPTG - J17886 - Alfa Aesar Alfa Aesar is a leading manufacturer and supplier of research chemicals, pure metals and materials for a wide span of applications. Cookies disclaimer can homeschoolers play sports in georgia
IPTG - Thermo Fisher Scientific
WebThermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene. X-Gal (5-bromo-4-chloro-3-indolyl-β-D … WebIPTG (isopropyl-β-D-thiogalactopyranoside) 99% dioxane-free Supplier: Thermo Scientific Chemicals Warning Synonyms: (2R,3R,4S,5R,6S)-2- (Hydroxymethyl)-6- (isopropylthio)tetrahydro-2H-pyran-3,4,5-triol Beta-galactosidase inducer Formula: C₉H₁₈O₅S MW: 238.31 g/mol Melting Pt: 109…115 °C Storage Temperature: Refrigerator MDL … WebCAS: 367-93-1 MDL: MFCD00063273 Thermo Scientific Pierce IPTG is isopropyl-β-D-thiogalactopyranoside for use with beta-galactosidase substrates such as X-Gal to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene. fithope