Krt5-cre
Web12 nov. 2014 · Krt5-CRE/ERT2 (011916-MU, MMRRC, USA) mice were crossed with Rosa26-loxP-STOP-loxP-lacZ mice (003309, Jackson Laboratory, USA) to generate … WebKRT5. General description of the gene and the encoded protein (s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project. Official gene symbol, which is typically a short form of the gene name, according to HGNC. Full gene name according to HGNC.
Krt5-cre
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Web1 jan. 2015 · Krt5+ cells appearing by day 11 post influenza infection were essentially completely untraced using CC10− or SPC-CreERT2 drivers (Fig. 1B–E, Extended Data … Web15 aug. 2024 · Rosa26-CreERT2 小鼠种系能够通过体内他莫昔芬诱导对floxed基因表达进行时间控制。. 也就是在Rosa26位点插入了CreERT2基因使其能能够稳定表达，当进行他莫昔芬Tamoxifen给药时，Cre重组酶从细胞质进入细胞核中，作用于loxp位点从而完成条件性基因敲除。. Rosa26位点是 ...
Web13 apr. 2024 · This context was mimicked by examining release from cytostatic doses of the dual ErbB inhibitor, lapatinib, in TM15 clone 6 (TM15c6) cells derived from an Erbb2-amplified tumor of a P MMTV-Cre ... Web26 sep. 2024 · Using a virus expressing Cre recombinase to permanently label infected cells in a Cre-inducible ... Samples were then stained with antibodies for active infection (HA, PY102), basal stem cells (Krt5, Biolegend, clone Poly19055, cat 905501), ciliated cells (FoxJ1, eBioscience, clone 2A5, cat 14–9965), secretory cells (SSEA-1 ...
Web图1. TdTomato的打靶策略[1] 这种鼠在正常情况下由于3×stop的存在并不能表达红色荧光，当与Cre鼠或TAM激活的CreERT2鼠交配后，产生的后代由于Cre重组酶切割loxp位点之间的序列将3×stop切除，TdTomato得到表达，就可以在Cre或CreERT2表达的组织中发出红色荧光，指示基因的位置。 Web1 nov. 2024 · Krt5 Cre ERT2-Gt (ROSA)26Sor tm4(ACTB-tdTomato-EGFP) mice were used to perform lineage tracing. For induction of Cre-ERT2 protein, 8–10-week-old mice were injected with tamoxifen dissolved in corn oil via an intra-peritoneal route. For each mouse, 0.1 mg tamoxifen was used to label single cells (Kusaba et al. 2014; Kang et al. 2016).
WebThe keratin 5-Cre recombinase transgenic mouse line has been used to generate skin specific gene deletions. Anatomical context of Tg(KRT5-cre)1Tak When the K5Cre (+/+) mammary glands were examined, we not only found a significant decrease in the number of mammary branches in the virgin females, but also a greater number of quiescent alveoli …
Web14 mrt. 2024 · Correspondingly, acute removal of Ch25h from keratinocytes (by Tam treatment of Krt5 Cre/ERT2;Ch25h fl/fl mice 39 also specifically diminished the frequency of Vγ2 + Tγδ17 cells (Figure S5 D). Together, these results show that basal keratinocytes express Ch25h that can be modulated by inflammatory cues and that the GPR183 … osu lazer editorWeb21 mrt. 2024 · KRT5 (Keratin 5) is a Protein Coding gene. Diseases associated with KRT5 include Epidermolysis Bullosa Simplex 2F, With Mottled Pigmentation and Epidermolysis … osu lazer score submissionWeb10 apr. 2024 · Krt5 urothelial cells are developmental and tissue repair progenitors in the kidney Ashley R. Jackson,1,2Monica L. Hoff,1,3Birong Li,1,2Christina B. Ching,1,2,4Kirk M. McHugh,1,2,5and X Brian Becknell1,2,3,6 1Nephrology Urology Research Affinity Group, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio; osu lazer install locationWebThe BK5.CreERT2 DNA construct was developed by fusing the cDNA for Cre recombinase to a mutated ligand binding domain of the human estrogen receptor (ER) resulting in … osu lacrosse ticketsWebFigure 1. Preliminary method for culturing alveolar organoids results in basal outgrowth. (A) Flow cytometry-based quantification of Sftpc-eGFP-expressing cells over time in organoid culture under preliminary culture conditions.A total of n = 2 independent lines was established from Sftpc-eGFP mice. (B) GSEA enrichment plots of AT2 and basal cell … osu lazer uninstallWeb证明Krt5- (IRES-CreERT2)小鼠可以成为实现Krt阳性细胞特异性敲除或表达的工具鼠。. 图 2 Tamoxifen 诱导后，Krt5 CreERT2/+; Rosa26 tdTomato/+ 小鼠各组织中tdTomato的表达 … osu lewis center imagingWeb29 jan. 2015 · Krt5-CRE/ERT2 (01191 6-MU, MMRRC, USA) micewere crossed wit h Rosa26-loxP-STOP-l oxP-lac Z mice (00 3309, Ja ckson. Laboratory, USA) to genera te mice for lineage tracing. Ge notype was confirmed. osul cortical